Mitochondria Antibody (AE-1), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24437100	Novus Biologicals NBP2443710.02MG	0.02 mg
NBP24437101	Novus Biologicals NBP2443710.1MG	0.1 mg
NBP24437102	Novus Biologicals NBP2443710.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Western Blot, ELISA, Immunohistochemistry (Paraffin), Immunofluorescence

Mitochondria Monoclonal specifically detects Mitochondria in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Mitochondria
• Concentration: 0.2mg/mL
• Applications: Western Blot, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cellular Markers, Mitochondrial Markers
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

• Clone: AE-1
• Dilution: Western Blot 0.25 - 0.5 ug/ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse (Negative), Rat (Negative)
• Immunogen: Semi-purified mitochondrial preparation
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 60 kDa

For Research Use Only