Invitrogen™ Mitotic Cells Monoclonal Antibody (8B3G)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA106200

Catalog No. MA106200

Description

MA1-06200 detects Mitotic cells in human samples. MA1-06200 has sucessfully been used in immunocytochemistry and FACS procedures. The MA1-06200 immunogen is total cell lysate of the human bladder carcinoma cell line T24.

The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques, such as the BrdU incorporation assay. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a quiescent phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S, and from G2 to M-phase.

Specifications

• Antigen: Mitotic Cells
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Immunogen: Total cell lysate of the human bladder carcinoma cell line T24.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry
• Clone: 8B3G
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Target Species: Human, Zebrafish
• Product Type: Antibody
• Isotype: IgM