Invitrogen™ MLCK Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA576982

Catalog No. PIPA576982

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

MLCK (MLCK2), now also known as MYLK3, is of the myosin regulatory light chain kinases which facilitates muscle contraction. MLCK, a member of the Ser/Thr protein kinase family, is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

Specifications

• Antigen: MLCK
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MYLK3
• Gene Alias: caMLCK; cardiac MLCK; cardiac-MLCK; cardiac-MyBP-C associated Ca/CaM kinase; cardiac-MyBP-C-associated Ca/CaM kinase; cardiac-specific myosin light chain kinase; D830007F02Rik; MLC kinase; MLCK; MLCK2; Mylk3; Myosin light chain kinase 3; putative myosin light chain kinase 3; putative myosin light chain kinase 3; myosin light chain kinase 3; RGD1305801
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 213435, 91807
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q32MK0, Q3UIZ8
• Gene Symbols: MYLK3
• Immunogen: Recombinant full length Human MLCK.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG