Invitrogen™ MLH1 Monoclonal Antibody (4C9C7)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA515431

Catalog No. PIMA515431

Description

MA5-15431 targets MLH1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples. The MA5-15431 immunogen is purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.. MA5-15431 detects MLH1 which has a predicted molecular weight of approximately 85kDa.

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

Specifications

• Antigen: MLH1
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: Ascites with 0.03% sodium azide
• Gene Accession No.: P40692
• Gene Symbols: Mlh1
• Immunogen: Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.
• Regulatory Status: RUO
• Gene ID (Entrez): 4292
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 4C9C7
• Conjugate: Unconjugated
• Gene: Mlh1
• Gene Alias: 1110035C23Rik; AI317206; AI325952; AI561766; COCA2; colon cancer, nonpolyposis type 2; DNA mismatch repair protein Mlh1; DNA mismatch repair protein Mlh1-like protein; FCC2; hMLH1; HNPCC; HNPCC2; I79_014127; MGC5172; mismatch repair protein 1; MLH1; MLH-1; mutL homolog 1; mutL homolog 1 (E. coli); mutL homolog 1, colon cancer, nonpolyposis type 2; mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli); mutL protein homolog 1
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Monkey
• Product Type: Antibody
• Isotype: IgG1