Invitrogen™ MLH1 Monoclonal Antibody (A9F7)

Catalog No.	Quantity
FEMA550627	100 μL

Catalog No. FEMA550627 Supplier Invitrogen™ Supplier No. MA550627

Description

Mouse Monoclonal Antibody

Positive control: HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, F9 cell lysate, RAW264.7 cell lysate, C6 cell lysate, A431 cell lysate, Daudi cell lysate, K-562 cell lysate, HL-60 cell lysate, SW480 cell lysate, human colon carcinoma tissue, mouse colon tissue, rat small intestine tissue. Predicted band size: 85 kDa Subcellular Location: Nucleus, Chromosome.

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

Specifications

• Antigen: MLH1
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: A9F7
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.1% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene: Mlh1
• Gene Accession No.: P40692, P97679, Q9JK91
• Gene Alias: 1110035C23Rik; AI317206; AI325952; AI561766; COCA2; colon cancer, nonpolyposis type 2; DNA mismatch repair protein Mlh1; DNA mismatch repair protein Mlh1-like protein; FCC2; hMLH1; HNPCC; HNPCC2; I79_014127; MGC5172; mismatch repair protein 1; MLH1; MLH-1; mutL homolog 1; mutL homolog 1 (E. coli); mutL homolog 1, colon cancer, nonpolyposis type 2; mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli); mutL protein homolog 1
• Gene Symbols: Mlh1
• Host Species: Mouse
• Immunogen: Recombinant protein within human MLH1 aa 452-751.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 17350, 4292, 81685
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b