MMP-9, Rabbit anti-Rat, Human, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma ABT54425UL

Catalog No. ABT54425UL

Description

Anti-MMP-9, Cat. No. ABT544, is a rabbit polyclonal antibody that detects Matrix Metalloroteinase-9 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Matrix metalloproteinase-9 (UniProt: P50282; also known as EC: 3.4.24.35, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB) is encoded by the Mmp9 gene (Gene ID: 81687) in rat. Matrix Metalloproteinases (MMPs) are a family of zinc metallo-endopeptidases secreted by cells and are responsible for much of the turnover of matrix components. MMP-9 is a secreted extracellular matrix metalloproteinase that exhibits a broad range of substrate specificity for native collagens including types IV, V, VII, and X as well as gelatin, proteoglycans, and elastin. It can exist as monomer or disulfide-linked homodimer. MMP-9 is synthesized with a signal peptide (aa 1-19) and a propeptide (aa 20-107) that are subsequently cleaved to produce active enzyme. Its activity can be inhibited by TIMP-1, which binds exclusively to pro-MMP-9 (Kd ∽35 nM). MMP-9 contain a fibronectin-like domain inserted into the catalytic domain, presumably to enhance substrate binding. MMP-9 also contains a collagen type-V-like domain, which may enhance substrate binding and specificity. MMP-9 contains a cysteine switch (aa 98-105) where the conserved cysteine binds the catalytic zinc ion and inhibits its enzyme activity. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Specifications

• Antigen: MMP-9
• Classification: Polyclonal
• Dilution: Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MMP-9 in rat spleen and human bone marrow tissues.
• Gene Alias: Matrix metalloproteinase-9;EC: 3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB
• Host Species: Rabbit
• Molecular Weight of Antigen: ∽85 kDa obsereved; 78.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Quantity: 25 μL
• Research Discipline: Cell Structure
• Test Specificity: This rabbit polyclonal antibody detects human and rat Matrix metalloproteinase-9. It targets an epitope within 16 amino acids from the N-terminal half.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: Mmp9
• Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of rat Matrix metalloproteinase-9.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Rat, Human
• Isotype: IgG