Invitrogen™ MMP15 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA532501

Catalog No. PIPA532501

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta tissue.

MMPs are a group of enzymes involved in matrix degradation. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT-MMPs exist as integral membrane proteins. MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectively contain a C-terminal transmembrane domain anchoring them to the cell surface. They have an 8 amino acid insert in their catalytic domain. MT1-MMP cleaves progelatinase A (MMP-2) and progelatinase B to their active forms. MT2-MMP plays a minor role in activation of pro-MMP-2 to its active form in breast carcinomas. Coexpression of MT1-MMP and MT2-MMp in advanced stages of breast carcinomas is indicative of possible involvement of MT2-MMP in its metastasis.

Specifications

• Antigen: MMP15
• Classification: Polyclonal
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: O54732, P51511
• Gene Symbols: Mmp15
• Immunogen: Synthetic peptide from the catalytic domain of mouse MMP-15 / MT2-MMP protein.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Conjugate: Unconjugated
• Gene: Mmp15
• Gene Alias: AI503551; matrix metallo protease; matrix metallopeptidase 15; matrix metallopeptidase 15 (membrane-inserted); matrix metalloproteinase 15; matrix metalloproteinase 15 (membrane-inserted); matrix metalloproteinase-15; Membrane type 2-MMP; membrane-type matrix metalloproteinase 2; Membrane-type-2 matrix metalloproteinase; MMP; Mmp15; MMP-15; MMPs; MT2MMP; MT2-MMP; MT-MMP 2; MTMMP2; SMCP-2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17388, 4324
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid