Invitrogen™ MNAT1 Polyclonal Antibody

Catalog No.	Quantity
PIPA579690	100 μg

Catalog No. PIPA579690 Supplier Invitrogen™ Supplier No. PA579690

Description

Rabbit Polyclonal Antibody

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human Hela whole cell, human A431 whole cell, human A375 whole cell, human MCF-7 whole cell, rat testis tissue, rat L6 whole cell, mouse testis tissue, mouse NIH/3T3 whole cell. IHC: mouse testis tissue, rat testis tissue, human testis tissue. ICC/IF: A431 cell. Flow: A431 cell.

Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires phosphorylation at Thr 160. The enzyme responsible for phosphorylation of Thr 160 in Cdk2 and also Thr 161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit, a regulatory subunit and a subunit of unknown function. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. This previously undescribed protein, now termed Mat1, has been cloned as a protein that associates with the cyclin H-Cdk7 nuclear complex at all stages of the cell cycle. Cyclin H-Cdk7-Mat1 complexes display kinase activity towards Cdk activation domains, and the carboxy terminus of RNA polymerase II. Mat1 appears to constitute the first example of an assembly factor, essential for the formation of an active Cdk-cyclin complex.

Specifications

• Antigen: MNAT1
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide
• Gene: Mnat1
• Gene Accession No.: P51948, P51949
• Gene Alias: AdoMet synthetase; AMS1; CAP35; CDK7/cyclin H assembly factor; CDK7/cyclin-H assembly factor; CDK-activating kinase assembly factor MAT1; cyclin G1 interacting protein; cyclin-G1-interacting protein; E130115E11Rik; EC 2.5.1.6; Mat1; MATA1; MAT-I/III; Menage a trois; menage a trois 1; menage a trois 1 (CAK assembly factor); menage a trois homolog 1, cyclin H assembly factor; menage a trois-like protein 1 cyclin H assembly factor; MNAT CDK-activating kinase assembly factor 1; MNAT1; MNAT1, CDK activating kinase assembly factor; p35; p36; RING finger protein 66; RING finger protein MAT1; RNF66; TFB3
• Gene Symbols: Mnat1
• Host Species: Rabbit
• Immunogen: E.coli-derived human MNAT1 recombinant protein (Position: L92-A278).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 17420, 266713, 4331
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov