Invitrogen™ MnSOD Monoclonal Antibody (MnS-1), eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ BMS122

Catalog No. 50-177-68

Description

Description: Recognizes human Mn SOD. Cross-reacts with rat and rabbit Mn SOD. The antibody can be used to stain both indirectly or directly acetone-fixed cryostat sections or cell smears. BMS122 is suitable for Western blot application. Under reducing conditions/SDS electrophoresis using BMS122 a 25 kDa band will be recognized (monomer of MnSOD protein). The native form of the protein with a molecular weight of 80 kDa will be recognized under non-reducing conditions. Human MnSOD is a tetrameric enzyme with four identical subunits each harboring a Mn+3 atom. MnSOD catalyzes the dismutation of two molecules of superoxide anion into water and hydrogen peroxide. Applications Tested: Immunohistochemistry, Western Blotting. Purity: >95%.

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

Specifications

• Antigen: MnSOD
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2-7.4
• Gene Accession No.: P04179
• Gene Symbols: SOD2
• Immunogen: Rec. Human MnSOD.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry, Western Blot
• Clone: MnS-1
• Conjugate: Unconjugated
• Gene: SOD2
• Gene Alias: I79_015272; indophenoloxidase B; IPO B; IPOB; IPO-B; manganese SOD; manganese superoxide dismutase; manganese-containing superoxide dismutase; mangano-superoxide dismutase; manganous superoxide dismutase; mitochondrial superoxide dismutase 2; Mn superoxide dismutase; MNSOD; Mn-SOD; MVCD6; similar to manganous superoxide dismutase; Sod2; Sod-2; sod2.L; sodMt; Superoxide dimutase 2, mitochondrial; superoxide dismutase; superoxide dismutase (Mn type); superoxide dismutase [Mn], mitochondrial; superoxide dismutase 2; superoxide dismutase 2 L homeolog; superoxide dismutase 2, mitochondrial; superoxide dismutase 2, mitochondrial L homeolog
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6648
• Content And Storage: 4°C
• Form: Liquid