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Invitrogen™ Amplex™ Acetylcholine/Acetlycholinesterase Assay Kit

Catalog No. A12217
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A12217 500 Assays
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Catalog No. A12217 Supplier Invitrogen™ Supplier No. A12217
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Description

Detect activity levels as low as 0.002U/mL in one hour

The Amplex Red Acetylcholine/Acetylcholinesterase Assay Kit provides an ultrasensitive method for detecting acetylcholinesterase (AChE) activity in a fluorescence microplate reader or fluorometer. AChE activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red reagent), a sensitive fluorogenic probe for hydrogen peroxide. AChE converts the acetylcholine substrate to choline, which is then oxidized by choline oxidase to betaine and hydrogen peroxide. In the presence of horseradish peroxidase, hydrogen peroxide reacts with the Amplex Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin.

  • Little interference from autofluorescence in most biological samples
  • Detect AChE activity levels as low as 0.002U/mL in one hour
  • Detect acetylcholine levels as low as 0.3μM using excess AChE
  • Achieve detection ranges of 0.3μM to 100μM acetylcholine
  • Format allows for multiple time point measurements
  • Designed for minimal autofluorescence interference
  • Custom assay design and packaging are also available

Drug Discovery and Development, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence Intensity
For Use With (Application) Acetylcholine/Acetlycholinesterase Assay
For Use With (Equipment) Fluorescence Microplate Reader
Product Type Amplex Red Assay Kit
Product Line Amplex
Quantity 500 Assays
Shipping Condition Room Temperature
Substrate Properties Molecular Substrate
Substrate Type HRP (Horseradish Peroxidase) Substrate, Acetylcholinesterase Substrate
Target Enzyme Acetylcholinesterase
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For Research Use Only. Not for use in diagnostic procedures.

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