Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Molecular Probes™ Amplex™ Acetylcholine/Acetlycholinesterase Assay Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Detect activity levels as low as 0.002U/mL in one hour

Manufacturer:  Invitrogen™ A12217

Catalog No. A12217

Add to cart



The Amplex Red Acetylcholine/Acetylcholinesterase Assay Kit provides an ultrasensitive method for detecting acetylcholinesterase (AChE) activity in a fluorescence microplate reader or fluorometer. AChE activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red reagent), a sensitive fluorogenic probe for hydrogen peroxide. AChE converts the acetylcholine substrate to choline, which is then oxidized by choline oxidase to betaine and hydrogen peroxide. In the presence of horseradish peroxidase, hydrogen peroxide reacts with the Amplex Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin.

  • Little interference from autofluorescence in most biological samples
  • Detect AChE activity levels as low as 0.002U/mL in one hour
  • Detect acetylcholine levels as low as 0.3µM using excess AChE
  • Achieve detection ranges of 0.3µM to 100µM acetylcholine
  • Format allows for multiple time point measurements
  • Designed for minimal autofluorescence interference
  • Custom assay design and packaging are also available

Drug Discovery and Development, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Order Info

Shipping Condition: Room temperature



500 assays
Fluorescence Intensity
500 assays
Store in freezer (-5° to -30°C); protect from light
Assay Kit
571 to 585nm
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.