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Invitrogen™ Amplex™ Acetylcholine/Acetlycholinesterase Assay Kit
Description
The Amplex Red Acetylcholine/Acetylcholinesterase Assay Kit provides an ultrasensitive method for detecting acetylcholinesterase (AChE) activity in a fluorescence microplate reader or fluorometer. AChE activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red reagent), a sensitive fluorogenic probe for hydrogen peroxide. AChE converts the acetylcholine substrate to choline, which is then oxidized by choline oxidase to betaine and hydrogen peroxide. In the presence of horseradish peroxidase, hydrogen peroxide reacts with the Amplex Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin.
- Little interference from autofluorescence in most biological samples
- Detect AChE activity levels as low as 0.002U/mL in one hour
- Detect acetylcholine levels as low as 0.3μM using excess AChE
- Achieve detection ranges of 0.3μM to 100μM acetylcholine
- Format allows for multiple time point measurements
- Designed for minimal autofluorescence interference
- Custom assay design and packaging are also available
Drug Discovery and Development, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Content And Storage | Store in freezer -5°C to -30°C and protect from light. |
| Detection Method | Fluorescence Intensity |
| For Use With (Application) | Acetylcholine/Acetlycholinesterase Assay |
| For Use With (Equipment) | Fluorescence Microplate Reader |
| Product Type | Acetylcholine⁄Acetlycholinesterase Assay |
| Product Line | Amplex |
| Quantity | 500 assays |
| Shipping Condition | Room Temperature |
| Substrate Properties | Molecular Substrate |
| Substrate Type | HRP (Horseradish Peroxidase) Substrate, Acetylcholinesterase Substrate |
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For Research Use Only. Not for use in diagnostic procedures.
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