Invitrogen™ Amplex™ Red Catalase Assay Kit

Catalog No.	Quantity
A22180	400 Assays

Catalog No. A22180 Supplier Invitrogen™ Supplier No. A22180

Description

For use with absorbance and fluorescence intensity techniques

• Provides sensitive and simple fluorometric method for detecting as little as 50mU/mL of catalase activity in purified system in 100μL assay volume

Drug Discovery and Development, Enzyme and Protein Activity Assays, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• Detection Method: Absorbance, Fluorescent Intensity
• For Use With (Application): Catalase Assay
• Product Type: Catalase Assay
• Product Line: Amplex
• Quantity: 400 Assays
• Shipping Condition: Room Temperature
• Substrate Properties: Chemical Substrate
• Substrate Type: HRP (Horseradish Peroxidase) Substrate, Catalase Substrate
• Target Enzyme: Catalase

Frequently Asked Questions (FAQs)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H₂O₂ oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H₂O₂ required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H₂O₂ in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.