Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Amplex™ Red Cholesterol Assay Kit

Catalog No. A12216
Encompass_Preferred
Click to view available options
Quantity:
500 assays

Includes

Amplex Red reagent, DMSO, Horseradish peroxidase, Hydrogen peroxide, Cholesterol esterase, Cholesterol oxidase, Cholesterol reference standard, Resorufin fluorescence reference standard, 5X Reaction buffer

Detects both free cholesterol and cholesteryl esters

Designed for minimal autofluorescence interference, the Amplex Red Cholesterol Assay Kit provides a sensitive, rapid, and simple fluorometric method for detecting very low concentrations of cholesterol using a fluorescence microplate reader or fluorometer.

  • Detects as little as 200nm (80ng/mL) cholesterol
  • Helps accurately measure cholesterol content of 0.01μL of human serum
  • Detects both free cholesterol and cholesteryl esters
  • Allows for multiple time point measurements
  • Designed for minimal autofluorescence interference
  • Custom assay design and packaging are also available

Cholesterol Measurement is Simple and Fast

  • Quick and easy assay, requiring only 30-minute incubation after addition of reagents with no separation steps required
  • Assay volumne of 100μL is designed for 96-well plates
  • Fluorescence can be measured with microplate reader or fluorometer
  • Since the assay is continuous, once the Amplex Red reagent is added, multiple time-point measurements can be taken
  • Complete kit with all necessary reagents except deionized water
Helps Accurately Detect Very Low Cholesterol Concentrations
  • Utilizes enzyme-coupled reactions to produce highly fluorescent resorufin for quantitation
  • Resorufin is produced by the reaction of the Amplex Red reagent with H2O2 produced from the cholesterol oxidase-catalyzed oxidation of cholesterol
  • Large proportion of cholesterol in blood is in the form of cholesteryl esters and hence cholesterol esterase is used to produce free cholesterol from cholesterol esters
  • Performing the assay in the presence and absence of cholesterol esterase potentially allows determination of the fractions of cholesterol in each form
Use Amplex Red Assays for a Broad Range of Investigations
Wide variety of validated Invitrogen Amplex Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex UltraRed, a second-generation reagent providing greater sensitivity and brighter fluorescence and the Amplex Red/UltraRed stop reagent. The Amplex Red/UltraRed stop reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least 3 hours.

Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Color Red
Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Cholesterol Assay
For Use With (Equipment) Microplate Reader
Product Type Red Cholesterol Assay Kit
Dye Type Amplex™ Red
Format 96-well plate
Product Line Amplex
Quantity 500 assays
Shipping Condition Room Temperature
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.