Invitrogen Molecular Probes Amplex Red Galactose/Galactose Oxidase Assay Kit
Detectis galactose or monitors galactose oxidase activity
Manufacturer: Invitrogen A22179
The Amplex Red Galactose/Galactose Oxidase Assay Kit provides a sensitive and simple method for detecting galactose oxidase activity or galactose using a fluorescence microplate reader or fluorometer. Galactose oxidase is an important tool in the detection of galactose in biological fluids. The Amplex Red Galactose/Galactose Oxidase Assay Kit provides an ultrasensitive method for detecting galactose or for monitoring galactose oxidase activity. Galactose oxidase catalyzes the oxidation of galactose at the C6 position and generates hydrogen peroxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide reacts in a 1:1 stoichiometric ratio with the Amplex Red reagent to generate the red-fluorescent compound resorufin. Because resorufin has absorption and fluorescence emission maxima of approximately 571nm and 585nm, respectively, there is little interference from autofluorescence in most biological samples.
- Detect concentrations as low as 2mU/mL of galactose oxidase activity or 4µM galactose
- Format allows for multiple time point measurements
- Designed for minimal autofluorescence interference
- Custom assay design and packaging are also available
Use Amplex Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex UltraRed Reagent, a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex Red/UltraRed Stop Reagent. The Amplex Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours.
Enzyme and Protein Activity Assays, Glycosidase, Neuraminidase, and Related Enzyme Activity, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis
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Shipping Condition: Room temperature
|96-well plate, Tube(s)|
|Store in freezer (-5° to -30°C) and protect from light.|
|Fluorometer, Microplate Reader, Spectrophotometer|
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For Research Use Only. Not for use in diagnostic procedures.