Invitrogen Molecular Probes Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit
Sensitive, one-step assay to detect hydrogen peroxide (H2O2) or peroxidase activity
Manufacturer: Invitrogen A22188
Assay uses AmplexRed reagent (10-acetyl-3,7-dihydroxyphenoxazine) to detect hydrogen peroxide (H2O2) or peroxidase activity in vitro
. AmplexRed reagent, in combination with horseradish peroxidase (HRP), can detect H2O2 released from biological samples, including cells, or generated in enzyme-coupled reactions. AmplexRed reagent can also be used as an ultrasensitive assay for peroxidase activity when H2O2 is in excess. In the presence of peroxidase, AmplexRed reagent reacts with H2O2 in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. Resorufin has excitation and emission maxima of approximately 571 nm and 585 nm, respectively, and because the extinction coefficient is high (58,000 ± 5000cm1M1), the assay can be performed fluorometrically or spectrophotometrically. This reaction has been used to detect as little as 10 picomoles of H2O2 in a 100µL volume or 1 x 105U/mL of HRP.
- Can detect as little as 10 picomoles of hydrogen peroxide or 10µU/mL of horseradish peroxidase activity in a 100µL assay volume
- Provides sufficient reagents for approximately 500 assays using reaction volumes of 100µL per assay
Cell Analysis, Cell Culture, Cell Viability, Proliferation and Function, Enzyme and Protein Activity Assays, Nitro-Oxidative Stress, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Reactive Oxygen Species, Stem Cell Analysis, Stem Cell Labeling and Detection, Stem Cell Research
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Shipping Condition: Room Temperature
|Store in freezer (-5° to -30°C) and protect from light.|
|96-well plate, Tube(s)|
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For Research Use Only. Not for use in diagnostic procedures.