Invitrogen™ Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay

Description

Includes

The kit contains sufficient reagents for performing two plates in a 96-well plate format.

Non-radioactive technique

The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or 'click' reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor 488 alkyne. The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min/Max format.

• Less hazardous

Cell Analysis, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function

Order Info

Shipping Condition: Room temperature

Specifications

• Color: Blue, Green
• Content And Storage: The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
  Store at ≤-20°C, desiccate and protect from light.
• Detection Method: Fluorescence
• For Use With (Application): High-content Screening (HCS)
• For Use With (Equipment): Fluorescence Microscope, High Content Analysis Instrument
• Product Type: HCS Assay
• Dye Type: Alexa Fluor™ 488, Hoechst 33258
• Format: 96-well plate
• Green Features: Less hazardous
• Product Line: Alexa Fluor, Click-iT, Molecular Probes
• Quantity: 2 plates
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.