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Invitrogen™ CoroNa™ Green, AM, cell permeant - Special Packaging

Catalog No. C36676
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C36676 20 x 50 μg
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Catalog No. C36676 Supplier Invitrogen™ Supplier No. C36676
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Description

A sodium ion indicator that exhibits an increase in green fluorescence emission intensity upon binding Na+, with little shift in wavelength

  • Allows spatial and temporal resolution of Na+ concentrations in presence of physiological concentrations of other monovalent cations
  • Dye loads into cells more efficiently than does sodium green tetraacetate, and CoroNa Green indicator responds to broader range of Na+ concentration
  • With absorption/emission maxima of approximately 492/516nm for Na+-bound form, can be detected by any instrument that can detect fluorescein

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Sodium and Potassium Detection

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Cell Viability and Proliferation
For Use With (Equipment) Fluorescence Microscope
Product Type Sodium Indicator
Dye Type Sodium Indicator
Product Line CoroNa
Quantity 20 x 50 μg
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.

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