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Invitrogen™ DiBAC4(3) (Bis-(1,3-Dibutylbarbituric Acid)Trimethine Oxonol)

Catalog No. B438
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B438 25 mg
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Catalog No. B438 Supplier Invitrogen™ Supplier No. B438
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Description

The slow-response potential-sensitive probe, DiBAC4(3) can enter depolarized cells where it binds to intracellular proteins or membrane and exhibits enhanced fluorescence and a red spectral shift.

The slow-response potential-sensitive probe, DiBAC4(3) can enter depolarized cells where it binds to intracellular proteins or membrane and exhibits enhanced fluorescence and a red spectral shift. Increased depolarization results in additional influx of the anionic dye and an increase in fluorescence. Conversely, hyperpolarization is indicated by a decrease in fluorescence. This bis-oxonal has an excitation maxima of 490 nm and emission maxima of 516 nm. DiBAC dyes are excluded from mitochondria because of their overall negative charge, making them superior to carbocyanines for measuring plasma membrane potentials.

Dissolve in high-quality anhydrous DMSO or ethanol to prepare a stock concentration up to 1 mM.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store at room temperature and protect from light.
Product Type DiBAC4(3)
Dye Type Membrane Potential Probes
Quantity 25 mg
Shipping Condition Room Temperature
Excitation/Emission 493/516 nm
Molecular Weight (g/mol) 516.64

Frequently Asked Questions (FAQs)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.
What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

For Research Use Only. Not for use in diagnostic procedures.

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