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Invitrogen™ EnzChek™ Ultra Amylase Assay Kit
Description
The EnzChek Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.
- Label (Ex/Em): BODIPY FL conjugate (approx. 502/512nm)
- Kit contains lyophilized substrate, 10X reaction buffer, substrate solvent, a fluorescent standard, and a detailed protocol
- Sufficient reagents are supplied for 500 assays (using a 100μL assay volume in a 96-well microplate assay format)
Find Fluorescent Substrates for Other Glycosidases
In addition to the EnzChek Ultra Amylase Assay Kit, we offer kits and substrates to measure xylanase, lysozyme, β-galactosidase, and more.
Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Glycosidase, Neuraminidase, and Related Enzyme Activity, High-Content Screening (HCS), Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Content And Storage | Store at room temperature and protect from light. |
| Detection Method | Fluorescence Intensity |
| For Use With (Application) | Ultra Amylase Assay |
| For Use With (Equipment) | Fluorescence Microplate Reader |
| Product Type | Amylase Assay |
| Product Line | EnzChek |
| Quantity | 1 Kit |
| Shipping Condition | Room Temperature |
| Substrate Properties | Molecular Substrate |
| Substrate Type | Amylase Substrate |
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For Research Use Only. Not for use in diagnostic procedures.
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