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Invitrogen™ EnzChek™ Lysozyme Assay Kit
Description
The EnzChek Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme activity in solution. The increase in fluorescence resulting from lysozyme activity is measured using a fluorometer or fluorescence microplate reader. The assay utilizes an innovative approach to measure lysozyme activity. Micrococcus lysodeikticus cell walls are used that have been extensively labeled with fluorescein causing quench of the fluorescence signal. Active lysozyme enzyme hydrolyzes the b-(1-4)-glucosidic linkages between the N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the mucopolysaccharide cell wall, relieving the quenching and yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.
- Detect lysozyme activity concentrations as low as 20U/mL
- Use standard fluorescein (FITC) excitation/emission settings
- Format allows for continuous measurement of lysozyme activity
Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Glycosidase, Neuraminidase, and Related Enzyme Activity, High-Content Screening (HCS), Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Content And Storage | Store in freezer -5°C to -30°C and protect from light. |
| Detection Method | Fluorescence Intensity |
| For Use With (Application) | Lysozyme Assay |
| For Use With (Equipment) | Fluorescence Microplate Reader |
| Product Type | Lysozyme Assay |
| Product Line | EnzChek |
| Quantity | 400 assays |
| Shipping Condition | Room Temperature |
| Substrate Properties | Lipid-Based Substrate |
| Substrate Type | Lysozyme Substrate |
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For Research Use Only. Not for use in diagnostic procedures.
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