Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. Cell Function Reagents and Kits
  6. E22013

Invitrogen™ EnzChek™ Lysozyme Assay Kit

Catalog No. E22013
Encompass_Preferred
Change view
Click to view available options
Quantity:
400 Assays
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
E22013 400 Assays
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. E22013 Supplier Invitrogen™ Supplier No. E22013
Only null left
Add to Cart
Edge
Add to Cart

Description

Measure lysozyme activity in solution

The EnzChek Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme activity in solution. The increase in fluorescence resulting from lysozyme activity is measured using a fluorometer or fluorescence microplate reader. The assay utilizes an innovative approach to measure lysozyme activity. Micrococcus lysodeikticus cell walls are used that have been extensively labeled with fluorescein causing quench of the fluorescence signal. Active lysozyme enzyme hydrolyzes the b-(1-4)-glucosidic linkages between the N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the mucopolysaccharide cell wall, relieving the quenching and yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.

  • Detect lysozyme activity concentrations as low as 20U/mL
  • Use standard fluorescein (FITC) excitation/emission settings
  • Format allows for continuous measurement of lysozyme activity

Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Glycosidase, Neuraminidase, and Related Enzyme Activity, High-Content Screening (HCS), Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence Intensity
For Use With (Application) Lysozyme Assay
For Use With (Equipment) Fluorescence Microplate Reader
Product Type EnzChek Assay Kit
Product Line EnzChek
Quantity 400 Assays
Shipping Condition Room Temperature
Substrate Properties Lipid-Based Substrate
Substrate Type Lysozyme Substrate
Target Enzyme Lysozyme
Show More Show Less

Frequently Asked Questions (FAQs)

How can I limit signal saturation when using the EnzChek Lysozyme Assay Kit (Cat. No. E22013)?

To limit signal saturation when using the EnzChek Lysozyme Assay Kit (Cat. No. E22013), try lowering the voltage (gain) on the PMT (photomultiplier tube). If you are still seeing signal saturation after lowering the voltage, try a shorter reaction time, and/or lower substrate concentration.

How was the substrate (Component A) in the EnzChek Lysozyme Assay Kit labeled?

The primary amines on the cell wall of Micrococcus lysodeikticus were conjugated to fluorescein.

May other buffers be used for the EnzChek Lysozyme assay?

Yes. You may use other buffers that are compatible with the enzyme of interest. The buffer provided is optimal for use with chicken egg white lysozyme. If the buffer has a pH <6, adjust the pH to 7.5 to 8 prior to detection for optimal signal from the fluorescein. The emission of fluorescein drops off at pH <6.

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.