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Invitrogen™ EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit

Description
Myeloperoxidase (MPO) is unique peroxidase that, in addition to its peroxidation activity, also catalyzes conversion of hydrogen peroxide (H2O2) and chloride (Cl-) to hypochlorous acid (HOCl). The EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit provides assays for determination of both chlorination and peroxidation activities of MPO in solution and in cell lysates.
- For detection of chlorination, kit includes nonfluorescent 3'-(p-aminophenyl) fluorescein (APF), which is selectively cleaved by hypochlorite (-OCl) to yield fluorescein Peroxidation is detected using nonfluorescent AmplexUltraRed reagent (A36006), which is oxidized by H2O2-generated redox intermediates MPO-I and MPO-II to form fluorescent product
- EnzChek Myeloperoxidase Activity Assay Kit can be used to continuously detect these activities at room temperature over broad dynamic range (1.5 to 200ng/mL)
- Kit combines speed (30 minutes), sensitivity and mix-and-read convenience
Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Nitro-Oxidative Stress, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Reactive Oxygen Species
Order Info
Shipping Condition: Wet Ice

Specifications
Specifications
Content And Storage | Store in refrigerator 2°C to 8°C and protect from light. |
Detection Method | Fluorescence Intensity |
For Use With (Application) | Myeloperoxidase (MPO) Activity Assay |
Product Type | Myeloperoxidase (MPO) Assay |
Product Line | EnzChek |
Quantity | 1 Kit |
Shipping Condition | Wet Ice |
Substrate Properties | Chemical Substrate |
Target Enzyme | Myeloperoxidase |
For Research Use Only. Not for use in diagnostic procedures.
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