Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. Cell Function Reagents and Kits
  6. E33856

Invitrogen™ EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit

Catalog No. E33856
Encompass_Preferred
Change view
Click to view available options
Quantity:
1 Kit
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
E33856 1 Kit
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. E33856 Supplier Invitrogen™ Supplier No. E33856
Add to Cart
Edge
Add to Cart

missing translation for 'validMainframeMsg'

Description

For measuring MPO activities and high-throughput screening for MPO-specific inhibitors

Myeloperoxidase (MPO) is a unique peroxidase that, in addition to its peroxidation activity, also catalyzes the conversion of hydrogen peroxide (H2O2) and chloride (Cl-) to hypochlorous acid (HOCl). The EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit provides assays for the determination of both chlorination and peroxidation activities of MPO in solution and in cell lysates. For detection of chlorination, the kit includes nonfluorescent 3'-(p-aminophenyl) fluorescein (APF), which is selectively cleaved by hypochlorite (-OCl) to yield fluorescein. Peroxidation is detected using nonfluorescent Amplex™ UltraRed reagent (A36006), which is oxidized by the H2O2-generated redox intermediates MPO-I and MPO-II to form a fluorescent product. The EnzChek™ Myeloperoxidase Activity Assay Kit can be used to continuously detect these activities at room temperature over a broad dynamic range (1.5 to 200 ng/mL). The speed (30 minutes), sensitivity, and mix-and-read convenience make this kit ideal for measuring MPO activities and for high-throughput screening for MPO-specific inhibitors.

Order Info

Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in refrigerator 2°C to 8°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Enzyme and Metabolic Activity
Product Type EnzChek Assay Kit
Product Line EnzChek
Quantity 1 Kit
Shipping Condition Wet Ice
Substrate Properties Chemical Substrate
Target Enzyme Myeloperoxidase
Excitation Wavelength 530 nm
Emission Wavelength 590 nm
Show More Show Less

Frequently Asked Questions (FAQs)

What is the dynamic range of the EnzChek Myeloperoxidase (MPO) Activity Assay Kit (Cat. No. E33856)?

The EnzChek Myeloperoixdase Activity Assay Kit (Cat. No. E33856) can detect myeloperoxidase activity at a broad dynamic range of 1.5 to 200 ng/mL.

What can interfere with the EnzChek Myeloperoxidase (MPO) Activity assay?

MPO is inhibited by azide, diclofenac, methimazole, quercetin, rutin, and salicylhydroxamic acid. Make certain that samples do not include sodium azide. Endogenous catalases can interfere with the assay; catalase activity may be inhibited by using 3-amino-1,2,4-triazole. Detergents, common components in cell lysis buffers, should be avoided; use freeze/thawing and/or mechanical methods to lyse cells.

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.