Invitrogen™ EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit

Description

For measuring MPO activities and high-throughput screening for MPO-specific inhibitors

Myeloperoxidase (MPO) is unique peroxidase that, in addition to its peroxidation activity, also catalyzes conversion of hydrogen peroxide (H₂O₂) and chloride (Cl^-) to hypochlorous acid (HOCl). The EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit provides assays for determination of both chlorination and peroxidation activities of MPO in solution and in cell lysates.

• For detection of chlorination, kit includes nonfluorescent 3'-(p-aminophenyl) fluorescein (APF), which is selectively cleaved by hypochlorite (^-OCl) to yield fluorescein Peroxidation is detected using nonfluorescent AmplexUltraRed reagent (A36006), which is oxidized by H₂O₂-generated redox intermediates MPO-I and MPO-II to form fluorescent product
• EnzChek Myeloperoxidase Activity Assay Kit can be used to continuously detect these activities at room temperature over broad dynamic range (1.5 to 200ng/mL)
• Kit combines speed (30 minutes), sensitivity and mix-and-read convenience

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Nitro-Oxidative Stress, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Reactive Oxygen Species

Order Info

Shipping Condition: Wet Ice

Specifications

• Content And Storage: Store in refrigerator 2°C to 8°C and protect from light.
• Detection Method: Fluorescence Intensity
• For Use With (Application): Myeloperoxidase (MPO) Activity Assay
• Product Type: Myeloperoxidase (MPO) Assay
• Product Line: EnzChek
• Quantity: 1 Kit
• Shipping Condition: Wet Ice
• Substrate Properties: Chemical Substrate
• Target Enzyme: Myeloperoxidase

For Research Use Only. Not for use in diagnostic procedures.