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Invitrogen™ EnzChek™ Phosphatase Assay Kit

Catalog No. E12020
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E12020 1000 Assays
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Catalog No. E12020 Supplier Invitrogen™ Supplier No. E12020
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Description

Continuous assay of prostatic acid phosphatase and protein phosphatase 1

Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes (proprietary) 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate. DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ∽1000 assays, using a reaction volume of 100μL per assay. Fluorescence (excitation/emission maxima approx. 358/455nm) can be measured in a fluorescence microplate reader or a standard fluorometer.

Cell Analysis, Cellular Imaging, Drug Discovery and Development, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Color Blue
Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Phosphatase Assay
For Use With (Equipment) Microplate Reader
Product Type EnzChek Assay Kit
Dye Type DiFMU
Emission 360/460
Product Line EnzChek
Quantity 1000 Assays
Shipping Condition Room Temperature
Substrate DiFMUP
Substrate Properties Chemical Substrate
Substrate Type Phosphatase Substrate
Target Enzyme Phosphatases
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For Research Use Only. Not for use in diagnostic procedures.

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