Invitrogen™ EnzChek™ Phosphate Assay Kit

Catalog No.	Quantity
E6646	100 Assays

Catalog No. E6646 Supplier Invitrogen™ Supplier No. E6646

Description

Determine phosphate released during ATPase or GTPase enzymatic reactions

The EnzChek™ Phosphate Assay Kit provides a fast and sensitive spectrophotometric method for the quantification of inorganic phosphate in solution and for phosphate released during ATPase or GTPase enzymatic reactions.

• Sensitivity of 2 to 150 μM phosphate
• Spectrophotometric microplate-based assay detects a change in absorbance maximum from 330 nm to 360 nm
• Continuous monitoring of phosphate generated by ATPase or GTPase activity

In the presence of inorganic phosphate, the 2-amino-6-mercapto-7-methylpurine riboside (MESG) substrate is converted by the purine nucleoside phosphorylase (PNP) enzyme to the ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine product. This enzymatic conversion of MESG results in a spectrophotometric shift in maximum absorbance from 330 nm for the substrate to 360 nm for the product.

Sensitivity of the assay is in the range of 2 to 150 μM inorganic phosphate (2 to 150 nanomoles phosphate in a 1 mL volume), and the reaction can be performed over a pH range of 6.5 to 8.5. The EnzChek™ phosphate reaction is sufficiently fast and quantitative for use in stopped-flow kinetic experiments.

Order Info

Shipping Condition: Room temperature

Specifications

• Color: Ultraviolet
• Content And Storage: Store in freezer -5°C to -30°C.
• Detection Method: Absorbance
• For Use With (Application): Phosphate Assay
• For Use With (Equipment): Spectrophotometer
• Product Type: EnzChek Assay Kit
• Dye Type: MESG
• Format: Tube(s)
• Product Line: EnzChek
• Quantity: 100 Assays
• Shipping Condition: Room Temperature
• Absorbance Wavelength: 360 nm

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.