Learn More
Invitrogen™ EnzChek™ Phosphate Assay Kit
Description
The EnzChek Phosphate Assay Kit provides a fast and sensitive spectrophotometric method for the quantification of inorganic phosphate in solution and for phosphate released during ATPase or GTPase enzymatic reactions. In the presence of inorganic phosphate, the 2-amino-6-mercapto-7-methylpurine riboside (MESG) substrate is converted by the purine nucleoside phosphorylase (PNP) enzyme to the ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine product. This enzymatic conversion of MESG results in a spectrophotometric shift in maximum absorbance from 330nm for the substrate to 360nm for the product. The reaction can be performed over a pH range of 6.5 to 8.5. The EnzChek phosphate reaction is sufficiently fast and quantitative for use in stopped-flow kinetic experiments.
- Sensitivity of 2 to 150μM phosphate
- Spectrophotometric microplate-based assay detects a change in absorbance maximum from 330nm to 360nm
- Continuous monitoring of phosphate generated by ATPase or GTPase activity
Anion Detection, Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Cellular Imaging, Drug Discovery and Development, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Ionic Homeostasis and Signaling, Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Color | Ultraviolet |
| Content And Storage | Store in freezer -5°C to -30°C. |
| Detection Method | Colorimetric |
| For Use With (Application) | Phosphate Assay |
| For Use With (Equipment) | Spectrophotometer |
| Product Type | Phosphate Assay |
| Dye Type | MESG |
| Format | Tube(s) |
| Product Line | EnzChek |
| Quantity | 100 assays |
| Show More |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.