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Invitrogen™ EnzChek™ Protease Assay Kit, green fluorescence, 100-1000 assays

Catalog No. E6638
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Catalog No. E6638 Supplier Invitrogen™ Supplier No. E6638
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Description

Continuously measure protease kinetics

The EnzChek™ Protease Assay Kit, green fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with fluorescence microplate reader, is proportional to protease activity.

  • Generalized mechanism allows for measurement of a variety of proteases
  • Can be used to continuously measure protease kinetics
  • Excitation/emission of 505/513 nm allows use of standard fluorescein (FITC) filter settings

This EnzChek™ Protease Assay Kit contains a casein derivative that has been extensively labeled with the pH-insensitive, green-fluorescent BODIPY™ FL dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY™ FL dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The green-fluorescent BODIPY™ FL dye has excitation and emission spectra similar to those of fluorescein.

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Enzyme and Metabolic Activity
For Use With (Equipment) Microplate Reader
Product Type EnzChek Assay Kit
Product Line EnzChek
Quantity 1 Kit
Research Category Differentiation
Shipping Condition Room Temperature
Substrate Properties Protein-Based Substrate
Substrate Type Protease Substrate
Target Enzyme Proteases
Excitation Wavelength 505 nm
Emission Wavelength 513 nm
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Frequently Asked Questions (FAQs)

What is the optimal pH range for protease activity detection using the EnzChek Protease Assay Kit?

The digestion buffer provided in the kit (pH 7.8) is recommended for detecting the protease activity of most proteolytic enzymes with activity optima from pH 7.4 to 8.0. However, if you are working with an enzyme that requires activation compounds or a unique pH environment, then prepare the specific buffer required in place of the digestion buffer.
What is the shelf life for EnzChek Protease Assay kits (Cat. Nos. E6638, E6639)?

As stated in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp06638.pdf) on page 2 under section "Storage and Handling", these kits are stable for 6-12 months if stored as indicated.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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