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Invitrogen™ EnzChek™ Protease Assay Kit, green fluorescence, 100-1000 assays
Description
The EnzChek Protease Assay Kit, green fluorescence, is a fast, simple and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with fluorescence microplate reader, is proportional to protease activity. This EnzChek Protease Assay Kit contains a casein derivative that has been extensively labeled with the pH-insensitive, green-fluorescent BODIPY FL dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY FL dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The green-fluorescent BODIPY FL dye has excitation and emission spectra similar to those of fluorescein.
- Generalized mechanism allows for measurement of a variety of proteases
- Can be used to continuously measure protease kinetics
- Excitation/emission of 505/513nm allows use of standard fluorescein (FITC) filter settings
Cell Analysis, Cell Culture, Cell Viability, Proliferation and Function, Differentiation and Development, Enzyme and Protein Activity Assays, Protease/Peptidase Activity, Proteins, Expression, Isolation and Analysis, Stem Cell Analysis, Stem Cell Differentiation, Stem Cell Protein Analysis, Stem Cell Research
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Content And Storage | Store in freezer -5°C to -30°C and protect from light. |
| Detection Method | Fluorescence Intensity |
| For Use With (Application) | Protease Assay |
| For Use With (Equipment) | Fluorescence Microplate Reader |
| Product Type | EnzChek Assay Kit |
| Product Line | EnzChek |
| Quantity | 1 Kit |
| Research Category | Differentiation |
| Shipping Condition | Room Temperature |
| Substrate Properties | Protein-Based Substrate |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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