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Invitrogen™ EnzChek™ Protease Assay Kit, green fluorescence, 100-1000 assays

Catalog No. E6638
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Catalog No. E6638 Supplier Invitrogen™ Supplier No. E6638
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Description

Continuously measure protease kinetics

The EnzChek Protease Assay Kit, green fluorescence, is a fast, simple and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with fluorescence microplate reader, is proportional to protease activity. This EnzChek Protease Assay Kit contains a casein derivative that has been extensively labeled with the pH-insensitive, green-fluorescent BODIPY FL dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY FL dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The green-fluorescent BODIPY FL dye has excitation and emission spectra similar to those of fluorescein.

  • Generalized mechanism allows for measurement of a variety of proteases
  • Can be used to continuously measure protease kinetics
  • Excitation/emission of 505/513nm allows use of standard fluorescein (FITC) filter settings

Cell Analysis, Cell Culture, Cell Viability, Proliferation and Function, Differentiation and Development, Enzyme and Protein Activity Assays, Protease/Peptidase Activity, Proteins, Expression, Isolation and Analysis, Stem Cell Analysis, Stem Cell Differentiation, Stem Cell Protein Analysis, Stem Cell Research

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence Intensity
For Use With (Application) Protease Assay
For Use With (Equipment) Fluorescence Microplate Reader
Product Type EnzChek Assay Kit
Product Line EnzChek
Quantity 1 Kit
Research Category Differentiation
Shipping Condition Room Temperature
Substrate Properties Protein-Based Substrate
Substrate Type Protease Substrate
Target Enzyme Proteases
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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