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Invitrogen™ EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays
Description
Detects metallo-, serine, acid, and sulfhydryl proteases.
The EnzChek Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with a fluorescence microplate reader, is proportional to protease activity. This EnzChek Protease Assay Kit contains a casein derivative that has been extensively labeled with the red-fluorescent BODIPY TR-X dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY TR-X dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The red-fluorescent BODIPY TR-X dye has excitation and emission spectra similar to those of the Texas Red fluorophore.
- Generalized mechanism allows for measurement of variety of proteases
- Can be used to continuously measure protease kinetics
- Excitation/emission similar to Texas Red™ dye (589/617nm)
Enzyme and Protein Activity Assays, Protease/Peptidase Activity, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Content And Storage | Store in freezer -5°C to -30°C and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Application) | Protease Assay |
| For Use With (Equipment) | Fluorescence Microplate Reader |
| Product Type | EnzChek Assay Kit |
| Product Line | EnzChek |
| Quantity | 1 Kit |
| Shipping Condition | Room Temperature |
| Substrate Properties | Protein-Based Substrate |
| Substrate Type | Protease Substrate |
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Frequently Asked Questions (FAQs)
The digestion buffer provided in the kit (pH 7.8) is recommended for detecting the protease activity of most proteolytic enzymes with activity optima from pH 7.4 to 8.0. However, if you are working with an enzyme that requires activation compounds or a unique pH environment, then prepare the specific buffer required in place of the digestion buffer.
As stated in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp06638.pdf) on page 2 under section "Storage and Handling", these kits are stable for 6-12 months if stored as indicated.
Reconstituted BODIPY casein substrates may be stored at 4 degrees C for 2-4 weeks. We recommend the addition of sodium azide at a final concentration of 2 mM to act as a preservative. If longer storage is required, freeze at -20 degrees C. Protect from light and avoid repeated freezing and thawing.
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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