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Invitrogen™ FluoZin™-2, AM, cell permeant
Description
Designed to detect Zn2+ concentrations that are present in synaptic vesicles and released in response to electrical stimulation or excitotoxic agonists
The FluoZin™-1 and FluoZin™-2 indicators are designed to detect Zn2+ concentrations that are present in synaptic vesicles and released in response to electrical stimulation or excitotoxic agonists. Based on the N-(2-methoxyphenyl)iminodiacetate chelator, these indicators are designed for detection of Zn2+ in the 0-100 μM range with minimal interfering Ca2+ sensitivity. FluoZin™ indicators are offered in cell-permeant and cell-impermeant forms.
Fluorescent Zinc Indicators Specifications:
- Label (Ex/Em): FluoZin™-2 (∼495/525 nm)
- Kd (Zn2+) (in buffer): ∼2 μM
- Lyophilized product may be dissolved in DMSO for use
- Product is typically loaded into cells by adding the dissolved indicator to medium containing cells
Find Fluorescent Indicators for Zinc and Other Metal Ions
We offer a number of fluorescent indicators to determine polyvalent cation concentrations inside cells, to follow metal ion transport through ion channels, to make measurements in environmental samples, and more. Review Fluorescent Indicators for Zn2+ and Other Metal Ions-Section 19.7 in the Molecular Probes™ Handbook for more information on these products.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Content And Storage | Store in freezer -5°C to -30°C and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Application) | Zinc Indicator |
| Product Type | Zinc Indicator |
| Dye Type | FluoZin-2 |
| Product Line | FluoZin |
| Quantity | 50 μg |
| Shipping Condition | Room Temperature |
| Excitation Wavelength | 495 nm |
| Emission Wavelength | 525 nm |
Frequently Asked Questions (FAQs)
Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.
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