Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

beta Galactosidase Rabbit anti-Bacteria, Polyclonal, Invitrogen™ Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen A11132

Catalog No. A11132


Add to cart

Description

Description

The E. coli gene lacZ encoding beta-galactosidase is an important reporter gene for detecting the expression of recombit genes in animal cells. In situations where enzymatic detection of beta-galactosidase is not practical, anti-beta-galactosidase antibody has been widely used. Anti-beta-galactosidase antibody can also be used for purification of fusion proteins by immunoprecipitation or immunoadsorption. Furthermore, anti-beta-galactosidase antibody has been shown to be the preferred analytical tool for light microscopic evaluation of lacZ gene transfer, and the beta-galactosidase/anti-beta-galactosidase complex has been used as a model to assess the feasibility of a high-pressure immunodesorption process. This antibody, raised in rabbit against E. coli beta-galactosidase, demonstrates high specificity for the enzyme and is suitable for immunological methods that require high titer and specificity such as immunoblotting (western or dot blot), ELISA, immunoprecipitation, and immunohistochemistry. The appropriate dilution of antibody for a particular application should be determined empirically. We suggest trying dilutions of about 1:5,000 to 1:50,000 for your initial experiments.

Beta-galactosidase is an exoglycosidase which hydrolyzes the beta-glycosidic bond formed between a galactose and its organic moiety. Deficiencies in the protein in humans can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of beta-galactosidase, the lacZ gene, is present as part of the inducible system lac operon, which is activated in the presence of lactose when glucose level is low. E. coli beta-galactosidase is commonly used in molecular biology as a reporter marker to monitor gene expression. Another popular use for beta-galactosidase is in blue/white screening to identify recombit clones. Beta-galactosidase can be split into two peptides, lacZalpha and LacZOmega, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme. This property is exploited in many cloning vectors. The presence or absence of an active beta-galactosidase may be detected through addition of artificial chromogenic substrates such as X-gal, fluorescent substrates such as Fluorescein di-beta-D-galactopyranoside (FDG), luminescent substrates, and others. Beta-galactosidase activity at pH 6 is an indicator of senescent cells not found in presenescent, quiescent or dividing cells.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Order Info

Shipping Condition: Wet Ice

Specifications

Specifications

beta Galactosidase
Polyclonal
Unconjugated
Liquid
Rabbit
IgG
500 μL
4° C
Primary
Bacteria
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
2 mg/mL
PBS with 5mM sodium azide; pH 7.2
Beta-Gal; ß-galactosidase; betagal; ß-gal; ßgal; bgal; beta-gal fusion protein; b-gal fusion protein
beta-Galactosidase
IgG fraction
RUO
Antibody
Polyclonal
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.