Invitrogen™ Escherichia coli (K-12 strain) BioParticles™, Alexa Fluor™ 488 conjugate

Description

Uniformly intense over the pH range from 3 to 10

• Conjugates covalently labeled with fluorophore (removed free dye after conjugation)
• Label (Ex/Em): Alexa Fluor488 (approx. 495/519nm)
• Particle: E. coli (K-12 strain)
• Conjugates are provided as lyophilized powders
• Approximately 3 x 10⁸ E. coli particles perm g solid and approximately 2 x 10⁷ zymosan particles permg solid
• Can be reconstituted in buffer of choice for use in phagocytosis assays
• Fluorescence of conjugates that are bound to surface of cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
• I addition to cellular applications, fluorescent conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
• Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Dry Ice

Specifications

• Cell Type: Mammalian Cell
• Content And Storage: Store in freezer (-5 to -30°C) and protect from light.
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescence Microscope
• Product Type: E.coli Conjugate
• Dye Type: Alexa Fluor™ 488
• Form: Solid
• Product Line: Alexa Fluor, BioParticles
• Quantity: 2 mg
• Shipping Condition: Dry Ice
• Species: E. coli

For Research Use Only. Not for use in diagnostic procedures.