Invitrogen™ Escherichia coli (K-12 strain) BioParticles™, Alexa Fluor™ 594 conjugate

Description

The fluorescence of the dye conjugates is uniformly intense over the pH range from 3 to 10.

Conjugates covalently labeled with fluorophores (special care has been taken to remove free dye after conjugation).

• Label (Ex/Em): Alexa Fluor594 (approx. 590/617nm)
• Conjugates are provided as lyophilized powders
• There are approximately 3 x 10⁸ E. coli or S. aureus particles per mg solid and approximately 2 x 10⁷ zymosan particles per mg solid
• Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
• Fluorescence of conjugates that are bound to surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
• In addition to cellular applications, fluorescent conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
• Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Store in freezer (-5 to -30°C) and protect from light.
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescence Microscope
• Product Type: E.coli Conjugate
• Dye Type: Alexa Fluor™ 594
• Form: Lyophilized Powder
• pH: 3 to 10
• Product Line: Alexa Fluor, BioParticles
• Quantity: 2 mg
• Shipping Condition: Room Temperature
• Species: E. coli

For Research Use Only. Not for use in diagnostic procedures.