Invitrogen™ Escherichia coli (K-12 strain) BioParticles™, fluorescein conjugate

Catalog No.	Quantity
E2861	10 mg

Catalog No. E2861 Supplier Invitrogen™ Supplier No. E2861

Description

The fluorescence of the dye conjugates is uniformly intense over the pH range from 3 to 10.

• Conjugates covalently labeled with variety of different fluorophores (special care has been taken to remove free dye after conjugation)
• Provided as lyophilized powders
• Approximately 3 x 10⁸ E. coli or S. aureus particles per mg solid and approximately 2 x 10⁷ zymosan particles per mg solid
• Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
• Fluorescence of conjugates that are bound to surface of cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
• Addition to cellular applications, fluorescent conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
• Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Store in freezer (-5 to -30°C) and protect from light.
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescence Microscope
• Product Type: E.coli Conjugate
• Dye Type: FITC (Fluorescein)
• Form: Solid
• pH: 3 to 10
• Product Line: BioParticles
• Quantity: 10 mg
• Shipping Condition: Room Temperature
• Species: E. coli

Frequently Asked Questions (FAQs)

How are the Escherichia coli (K-12 strain) BioParticles, fluorescein conjugate supplied?

The Escherichia coli (K-12 strain) BioParticles, fluorescein conjugate are supplied as lyophilized powder in a 10 mg.unit size. There are approximately 3 x 10E8 E. coli particles per mg solid.

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.

What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

What can the BioParticles product line be used for?

Fluorescent Bioparticles have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E.Coli, S. aureus, and zymosan BioParticles conjugates covalently labeled with a variety of different fluorophores.

For Research Use Only. Not for use in diagnostic procedures.