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Invitrogen™ Staphylococcus aureus (Wood strain without protein A) BioParticles™, Alexa Fluor™ 594 conjugate
Description
Fluorescent products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry
Unlike the fluorescence of fluorescein-labeled BioParticles conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10.
- Label (Ex/Em): Alexa Fluor 594 (approx. 590/617nm)
- Particle: S. aureus (Wood strain, without protein A)
- Provided as lyophilized powders
- approximately 3 x 108 S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid
- Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
- Conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
- Fluorescent BioParticles conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
- Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways
Cell Analysis, Cell Structure, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Receptors
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Equipment) | Fluorescence Microscope |
| Product Type | Bioparticle Conjugate |
| Dye Type | Alexa Fluor™ 594 |
| Form | Lyophilized Powder |
| pH | 3 to 10 |
| Product Line | Alexa Fluor, BioParticles |
| Quantity | 2 mg |
| Shipping Condition | Room Temperature |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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