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Invitrogen™ Staphylococcus aureus (Wood strain without protein A) BioParticles™, Alexa Fluor™ 594 conjugate

Catalog No. S23372
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S23372 2 mg
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Catalog No. S23372 Supplier Invitrogen™ Supplier No. S23372
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Description

Fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities

Fluorescent products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry

Unlike the fluorescence of fluorescein-labeled BioParticles conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10.

  • Label (Ex/Em): Alexa Fluor 594 (approx. 590/617nm)
  • Particle: S. aureus (Wood strain, without protein A)
  • Provided as lyophilized powders
  • approximately 3 x 108 S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid
  • Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
  • Conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
  • Fluorescent BioParticles conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
  • Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Cell Analysis, Cell Structure, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Receptors

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope
Product Type Bioparticle Conjugate
Dye Type Alexa Fluor™ 594
Form Lyophilized Powder
pH 3 to 10
Product Line Alexa Fluor, BioParticles
Quantity 2 mg
Shipping Condition Room Temperature
Species S. aureus
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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