Invitrogen™ JC-9 Dye (Mitochondrial Membrane Potential Probe)

Catalog No.	Quantity
D22421	5 mg

Catalog No. D22421 Supplier Invitrogen™ Supplier No. D22421

Description

Mitochondrial membrane potential probe

The ratiometric mitochondrial membrane potential probe JC-9 exhibits green fluorescence that is independent of membrane potential and an increase in red fluorescence at hyperpolarized membrane potentials.

Apoptosis, Cell Analysis, Cell Structure, Cell Viability & Cytotoxicity, Cell Viability, Proliferation & Function, Mitochondria, Mitochondria Function, Mitochondrial Function

Order Info

Shipping Condition: Room Temperature

Specifications

• Color: Green
• Content And Storage: Store at room temperature and protect from light.
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
• Product Type: Mitochondrial Membrane Potential Probe
• Quantity: 5 mg
• Shipping Condition: Room Temperature
• Sub Cellular Localization: Mitochondria

Frequently Asked Questions (FAQs)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

For Research Use Only. Not for use in diagnostic procedures.