Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Low Density Lipoprotein from Human Plasma (LDL)

Catalog No. L3486
Encompass_Preferred
Click to view available options
Quantity:
200 μL

Unlabeled LDL used to study normal cholesterol delivery and internalization

Human low-density lipoprotein (LDL) is large protein complex (approx. 500,000 Da) that binds to specific receptor on surface of vertebrate cells and delivers cholesterol via receptor-mediated endocytosis. Experiments to study LDL are typically performed by adding fluorescently labeled LDL to cultured cells and analyzing them by microscopy or flow cytometry. Alternatively the fluorescently labeled LDL can be injected into test animals, and the distribution of the label can be analyzed after the specified time period.

  • Label (Ex/Em): Unlabeled
  • Acetylated: No
  • Amount: 200 μL (2.5mg/mL)
Fresh LDL Produced Continually
  • We prepare our LDL and AcLDL products from fresh human plasma approximately every two months
  • Nonacetylated LDL products are shipped within two weeks of their preparation
  • All acetylated LDL products are available on a continuous basis
Nonacetylated vs. Acetylated LDL
  • LDL containing an unmodified apoprotein is used to study normal cholesterol delivery and internalization
  • If the lysine residues of LDL«s apoprotein have been acetylated, the LDL complex no longer binds to the LDL receptor, but rather is taken up by endothelial and microglial cells that possess “scavenger” receptors specific for that modified form
Key Applications for Labeled LDL
  • Applications for labeled LDL complexes include:
  • Counting cell-surface LDL receptors, and analyzing their motion and clustering following internalization
  • Quantitating LDL receptor activity in fibroblasts (replacing the radiolabeled LDL assay)
  • Investigating LDL expression and identifying LDL receptor deficiencies in various cell lines

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection

Order Info

Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY

Specifications

Concentration 2.5 mg⁄ml
Content And Storage Store in refrigerator (2–8°C).
Detection Method Fluorescence
Product Type Supplement
Dye Type Unlabeled
Quantity 200 μL
Shipping Condition Wet Ice
WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.