Invitrogen™ NeuroTrace™ Multicolor Tissue-Labeling Kit - DiO, DiI, DiD pastes, 500 mg each

Catalog No.	Quantity
N22884	1 kit

Catalog No. N22884 Supplier Invitrogen™ Supplier No. N22884

Description

Contains one paste each of DiI, DiO or DiD mixed into an inert, water-resistant gel

• Ready to use as supplied and can be applied directly to live or fixed tissue specimens using the tip of a needle
• Improves penetration of dye into bundled neurons, labeling axons both on and below surface

Cell Analysis, Cell Tracing and Tracking, Cellular Imaging, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Neuronal Tracing

Order Info

Shipping Condition: Room temperature

Specifications

• Color: Green, Yellow, Red
• Detection Method: Fluorescence
• Product Type: Tissue Labeling Kit
• Quantity: 1 kit
• Content And Storage: Store at room temperature and protect from light.
• Shipping Condition: Room Temperature
• For Use With (Equipment): Fluorescence Microscope
• Sub Cellular Localization: Cell Membranes & Lipids
• Product Line: NEUROTRACE

Frequently Asked Questions (FAQs)

I am doing retrograde neuronal labeling using the NeuroTrace DiI Paste. I apply a small glob onto the neuron of interest, but it is not adhering very well. What can I do?

This paste, which has DiI C₁₈ uniformly dispersed within a proprietary hydrophobic carrier, is commonly used to dab onto larger areas than smaller areas commonly used with crystals. Any moisture on the area the paste is applied may be the cause for lack of adherence. Apply the paste using a small flat-head spatula or toothpick and keep it in place a bit longer to ensure contact. The area may also be covered with parafilm to ensure contact as well.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.