Invitrogen™ Newport Green™ DCF Diacetate, cell permeant

Description

Has moderate zinc-binding affinity (K_d for Zn²⁺ approximately 1μM) but is essentially insensitive to Ca²⁺

• Kd for Ca²⁺ >100μM), making this valuable probe for detecting Zn²⁺ influx into neurons through voltage- or glutamate-gated channels
• When used alongside dyes with dual Ca²⁺ / Zn²⁺ sensitivity such as fura-2 and mag-fura-2, provides confirmation that changes in Zn²⁺ levels, and not Ca²⁺ or Mg²⁺ are being detected
• The response to transition metals, including Zn, Mn, Fe, Co, Cu(I), Cu(II), Ni and Cd, has been characterized
• Label (Ex/Em): Newport Green DCF (approximately 505/535nm)
• K_d (Zn²⁺) (in buffer): approximately 1μM
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding dissolved indicator to medium containing cells

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Zinc and Heavy Metal Ion Detection

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Store in freezer -5°C to -30°C.
• Detection Method: Fluorescence
• For Use With (Application): Cell Viability and Proliferation
• For Use With (Equipment): Fluorescence Microscope
• Product Type: DCF Diacetate
• Dye Type: Zinc Indicator
• Product Line: Newport Green
• Quantity: 1 mg
• Shipping Condition: Room Temperature

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.