Invitrogen™ Oregon Green™ 488 BAPTA-1, AM, cell permeant - Special Packaging

Description

Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca²⁺. They have uses in many calcium signaling investigations, including measuring intracellular Ca²⁺, following Ca²⁺ influx and release, and multiphoton excitation imaging of Ca²⁺ in living tissues.

• Fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry
• Label (Ex/Em): Oregon Green 488 BAPTA-1 (494/523nm)
• Fluorescence intensity increase upon binding Ca²⁺: approximately 14 fold
• Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength
• These probes are excited by visible light, and because energy required for excitation is low, potential for cellular photodamage is reduced
• Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of spectrum where cellular autofluorescence and scattering backgrounds are often less of problem

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Cell-Based Second Messenger Assays, Drug Discovery and Development, G-Protein Coupled Receptor Biology, Ionic Homeostasis and Signaling, Target and Lead Identification and Validation

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Store in freezer -5°C to -30°C.
• Detection Method: Fluorescence
• For Use With (Application): Cell Viability and Proliferation
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Microplate Reader
• Product Type: Stain
• Dye Type: Fluorescent Dye-Based
• Product Line: Oregon Green
• Quantity: 10 x 50 μg
• Shipping Condition: Room Temperature

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.