Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ PBFI, Tetraammonium Salt, cell impermeant

PBFI is potassium-sensitive molecule used measure intracellular K+ fluxes in animal cells and in plant cells and vacuoles

Manufacturer:  Invitrogen™ P1265MP

Encompass

Catalog No. P1265MP


Add to cart

Description

Description

  • Although selectivity of PBFI for K+ is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of K+ in presence of other monovalent cations
  • Spectral response of PBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with same optical filters and equipment used for fura-2
  • Label (Ex/Em): PBFI (approximately 340,380/500nm)
  • Lyophilized product may be dissolved in distilled water or aqueous buffer for use
  • Product is typically loaded into cells by diffusion from patch pipet for correlated fluorescence imaging and electrophysiological recording
  • Kd of PBFI for K+ is strongly dependent on whether Na+ is present, with value of 5.1mm in absence of Na+ and 44mm in solutions with combined Na+ and K+ concentration of 135mm (which approximates physiological ionic strength)
  • Although PBFI is only 1.5-fold more selective for K+ than for Na+, this selectivity is often sufficient because intracellular K+ concentrations are normally about 10 times higher than Na+ concentrations

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Sodium and Potassium Detection

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

Fluorescence Microscope
Fluorescent
Potassium Indicator
Room Temperature
Safety and Handling

Safety and Handling

SDS
Documents
Product Certifications
Promotions

Promotions

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.