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Invitrogen™ Rhod-2, AM, cell permeant

Catalog No. R1245MP
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20 x 50 μg
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R1245MP 20 x 50 μg
R1244 1 mg
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Description

Cells may be loaded with AM ester forms of these calcium indicators by adding dissolved indicator directly to dishes containing cultured cells

Labeled calcium indicators are molecules that exhibit increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring Ca2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca2+ release generated by photoreceptors and photoactivatable chelators.

  • Fluorescence signal from these cells is generally measured using fluorescence microscopy
  • Label (Ex/Em of Ca2+-bound form): Rhod-2 (552/581nm)
  • Fluorescence intensity increase upon binding Ca2+: >100 fold
  • Kd for Ca2+ in the absence of Mg2+, in buffer: approximately 570nM
  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
  • In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
  • Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Cell Viability and Proliferation
For Use With (Equipment) Fluorescence Microscope
Product Type Stain
Dye Type Fluorescent Dye-Based
Quantity 20 x 50 μg
Shipping Condition Room Temperature

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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