Invitrogen™ Rhodamine 123, 25 mg

Catalog No.	Quantity
R302	25 mg

Catalog No. R302 Supplier Invitrogen™ Supplier No. R302

Description

Cell-permeant, cationic, green-fluorescent dye

Rhodamine 123 is readily sequestered by active mitochondria without cytotoxic effects. This product has been used to assay mitochondrial membrane potential in populations of apoptotic cells.

Apoptosis, Cell Analysis, Cell Structure, Cell Viability & Cytotoxicity, Cell Viability, Proliferation & Function, Mitochondria, Mitochondria Function, Mitochondrial Function, Mitochondrial Structure

Order Info

Shipping Condition: Room Temperature

Specifications

• Color: Green
• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescence Microscope
• Product Type: Rhodamine 123
• Quantity: 25 mg
• Shipping Condition: Room Temperature
• Sub Cellular Localization: Mitochondria

Frequently Asked Questions (FAQs)

What is the excitation and emission wavelength for rhodamine?

Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (“EC”; cm^-1 M^-1), for various rhodamine dyes (data derived with dye dissolved in methanol).

Dye	Excitation	Emission	EC
Rhodamine B	568	583	88,000
Rhodamine 123	507	529	101,000
Rhodamine 110	499	521	92,000
Rhodamine 6G	528	551	105,000
XRITC	572	596	92,000

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.