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Invitrogen™ Transferrin From Human Serum, Alexa Fluor™ 555 Conjugate

Catalog No. T35352
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Quantity:
5 mg

Description

Labeled LDL complexes useful in studying the delivery of up to two Fe3+ atoms via receptor-mediated endocytosis

Transferrin is a monomeric serum glycoprotein (approx. 80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis.

  • Once iron-carrying transferrin proteins are inside endosomes, acidic environment favors dissociation of iron from the transferrin-receptor complex
  • Following release of iron, apotransferrin is recycled to plasma membrane, where it is released from its receptor to scavenge more iron
  • Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways
  • Experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy
  • Label (Ex/Em): Alexa Fluor 594 (590/617)
  • Amount: 15mg solid (contains 5mg of transferrin conjugate)
  • Applications include: Image transferrin receptor dynamics using FRET; Observe receptor trafficking in live cells by confocal laser-scanning microscopy; Investigate events occurring during endosomal acidification; Measure transferrin receptor binding affinity in mammals and parasites

Analysis of Bacteria, Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection, LPS Binding, Microbiological Analysis

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Detection Method Fluorescence
Product Type Transferrin
Dye Type Alexa Fluor Dyes
Form Solid
pH 7.2
Product Line Alexa Fluor
Quantity 5 mg
Shipping Condition Room Temperature
Excitation/Emission 555/565
Protein Family Transferrin
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For Research Use Only. Not for use in diagnostic procedures.

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