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Invitrogen™ Vybrant™ Phagocytosis Assay Kit

Catalog No. V6694
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Catalog No. V6694 Supplier Invitrogen™ Supplier No. V6694
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Description

Observe and quantitate process of phagocytosis in human polynuclear cells and mouse macrophages by following internalization of a foreign particle

  • Foreign particle is killed, E. coli (K-12 strain) cells that have been labeled with fluorescent dye fluorescein
  • Addition to lyophilized E. coli BioParticles™ component, kit contains a trypan blue solution (to quench fluorescence from particles that were not internalized) as well as step-by-step instructions for performing this phagocytosis assay in a fluorescence microplate reader
  • Methodology used with this kit has been developed using an adherent murine macrophage cell line (J774); however, by modifying the cell culture conditions, researchers can adapt this phagocytosis assay to other adherent cell lines
  • Particles are fluorescein-labeled E. coli (K-12 strain)
  • Kit supplies sufficient reagents for approx. 250 tests using 96-well plates
  • Fluorescence is measured using a microplate reader (Ex/Em approx. 480/520nm)

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Product Type Phagocytosis Assay Kit
Dye Type Classic Dyes
Format 96-well plate
Product Line Vybrant
Quantity 1 kit
Shipping Condition Room Temperature
Species E. coli

For Research Use Only. Not for use in diagnostic procedures.

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