Invitrogen™ Zymosan A (S. cerevisiae) BioParticles™, Alexa Fluor™ 488 conjugate

Catalog No.	Quantity
Z23373	2 mg

Catalog No. Z23373 Supplier Invitrogen™ Supplier No. Z23373

Description

Study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry

• Fluorescence of fluorescein-labeled BioParticles conjugates, partially quenched in acidic environments, fluorescence of Alexa Fluor, BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10
• Label (Ex/Em): Alexa Fluor488 (approx. 495/519nm)
• Particle: Zymosan (S. cerevisiae)
• Provided as lyophilized powders
• Approximately 3 x 10⁸ E. coli or S. aureus particles/mg solid and approximately 2 x 10⁷ zymosan particles/mg solid
• Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
• Fluorescence of BioParticles conjugates that are bound to surface of cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
• Addition to cellular applications, fluorescent BioParticles conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
• Small particles may be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Store in freezer (-5 to -30°C) and protect from light.
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescence Microscope
• Product Type: Supplement
• Dye Type: Alexa Fluor™ 488
• Form: Lyophilized Powder
• pH: 3 to 10
• Product Line: Alexa Fluor, BioParticles
• Quantity: 2 mg
• Shipping Condition: Room Temperature
• Species: S. cerevisiae

Frequently Asked Questions (FAQs)

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.

What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

What can the BioParticles product line be used for?

Fluorescent Bioparticles have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E.Coli, S. aureus, and zymosan BioParticles conjugates covalently labeled with a variety of different fluorophores.

What is the fluorescence excitation/emission of AlexaFluor 488 in the Zymosan A Bioparticles kit?

The fluorescence excitation/emission of AlexaFluor 488 in buffer at pH 8 is 495/519 nm.

For Research Use Only. Not for use in diagnostic procedures.