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Invitrogen™ Zymosan A (S. cerevisiae) BioParticles™, Alexa Fluor™ 488 conjugate

Catalog No. Z23373
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Z23373 2 mg
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Catalog No. Z23373 Supplier Invitrogen™ Supplier No. Z23373
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Description

Study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry

  • Fluorescence of fluorescein-labeled BioParticles conjugates, partially quenched in acidic environments, fluorescence of Alexa Fluor, BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10
  • Label (Ex/Em): Alexa Fluor488 (approx. 495/519nm)
  • Particle: Zymosan (S. cerevisiae)
  • Provided as lyophilized powders
  • Approximately 3 x 108 E. coli or S. aureus particles/mg solid and approximately 2 x 107 zymosan particles/mg solid
  • Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
  • Fluorescence of BioParticles conjugates that are bound to surface of cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
  • Addition to cellular applications, fluorescent BioParticles conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
  • Small particles may be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope
Product Type Supplement
Dye Type Alexa Fluor™ 488
Form Lyophilized Powder
pH 3 to 10
Product Line Alexa Fluor, BioParticles
Quantity 2 mg
Shipping Condition Room Temperature
Species S. cerevisiae
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For Research Use Only. Not for use in diagnostic procedures.

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