Invitrogen™ IL-8 Monkey ELISA Kit

For the quantitative detection of monkey IL-8.

Supplier:  Invitrogen™ BMS6403

Catalog No. 50-183-70

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to monkey IL-8
Biotin-Conjugate anti-monkey IL-8 polyclonal antibody
Streptavidin-HRP
Monkey IL-8 Standard Concentrate, 125000 U/ml
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

The Monkey Interleukin 8 (Mk IL-8) ELISA quantitates Mk IL-8 in cynomolus and baboon monkey serum or cell culture medium. The assay will exclusively recognize both natural and recombinant monkey IL-8. Principle of the method The Monkey IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

Specifications

• Assay Range: 19-1,250 U/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 100381196, 102127272
• Interassay CV: 8.7%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Serum, 50 μL; Supernatant, 50 μL
• Synonym: Protein 3-10C, CXCL8, NAP-1, GCP-1
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 3.0 U/mL
• Product Type: ELISA Kit
• Target Species: Monkey
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL8
• Intraassay CV: 6.3%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Baboon, Cynomolgus Monkey
• Total Assay Time: 3 hr. 10 min.

For Research Use Only