Invitrogen™ Mouse Angiopoietin-2 His-tag Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (705 μL) be added to the vial to prepare a stock solution of 0.71 mg/mL. Concentration is measured by UV.

ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich coiled coil N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.

Specifications

• Accession Number: O35608
• For Use With (Application): ELISA
• Format: Lyophilized
• Formulation: PBS with 0.01% mannitol, 0.01% Tween 80, 5-8% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 11601
• Molecular Weight (g/mol): 33.4 kDa
• Name: Mouse Angiopoietin-2 His-tag
• Purification Method: SDS-PAGE
• Quantity: 500 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: AGPT2; Ang2; Ang-2; angiopoietin 2; angiopoietin-2; angiopoietin-2 A; angiopoietin-2 B; ANGPT2; endothelial growth factor; Tie2-ligand
• Common Name: Angiopoietin 2
• Gene Symbol: ANGPT2
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Angiopoietin-2-His (at 2 μg/ml (100 μL/well) can bind mouse TEK-Fc, the EC50 of mouse TEK-Fc is 350-900 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Mouse ANGPT2, amino acids Lys275-Phe496 (Accession # NP_031452.2) with an N-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Angiopoietin-2-His (at 2 μg/ml (100 μL/well) can bind mouse TEK-Fc, the EC50 of mouse TEK-Fc is 350-900 ng/mL)
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.