MilliporeSigma™ Mouse anti-3-PGDH, Clone: TK-08 13A8,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1145

Catalog No. MABS1145MI

Description

Specifically detects 3-PGDH clone: TK-08 13A8 in Human samples, and it is validated for Western Blotting

D-3-phosphoglycerate dehydrogenase (EC1.1.1.95; UniProt O43175; also known as 3-PGDH, Epididymis secretory protein Li 113) is encoded by the PHGDH (also known as 3PGDH, HEL-S-113, NLS, NLS1, PGAD, PGDH, PDG, PGD, PGDH3, PHGDHD, SERA) gene (Gene ID 26227) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential targets for cancer treatment. L-serine is an important precursor for macromolecule biosynthesis. 3-PGDH catalyzes the first and rate-limiting step of the serine synthesis pathway from 3-phosphoglycerate to phospho-hydroxypyruvate and is important during tumor development. The PHGDH gene is frequently amplified and the enzyme is overexpressed in 70% of the estrogen receptor-negative breast cancers, 39% of melanoma, and cervical cancers.

Specifications

• Antigen: 3-PGDH
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: PHGDH; PGDH3
• Immunogen: Recombinant protein corresponding to human 3-PGDH.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_006614
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG1 κ

• Applications: Western Blot
• Clone: TK-08 13A8
• Gene Accession No.: O43175
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

For Research Use Only