MilliporeSigma™ Mouse anti-Active-β-Catenin, Clone: 8E7, Alexa Fluor 488,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 05665AF488

Catalog No. 05-665-AF488

Description

Specifically detects Active-β-Catenin clone: 8E7 in Human, Mouse samples, and it is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Catenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell–cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a 'destruction complex' with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.

Specifications

• Antigen: Active-β-Catenin
• Classification: Monoclonal
• Conjugate: Alexa Fluor 488
• Gene Accession No.: P35222
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Form: Purified

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry
• Clone: 8E7
• Formulation: Purified mouse conjugate antibody in PBS with 15mg/mL BSA and 0.1% sodium azide.
• Gene Symbols: CTNNB1; CTNNB; PRO2286
• Immunogen: Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag.
• Quantity: 100 μL
• Research Discipline: Cell Structure
• Gene ID (Entrez): NP_001895
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG1 κ

For Research Use Only