MilliporeSigma™ Mouse anti-Active-β-Catenin, Clone: 8E7, Alexa Fluor 647,
Mouse Monoclonal Antibody
Manufacturer: MilliporeSigma™ 05665AF647
Specifically detects Active-β-Catenin clone: 8E7 in Human, Mouse samples, and it is validated for Flow Cytometry, ImmunocytochemistryCatenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell–cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a 'destruction complex' with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.
|Purified mouse conjugate antibody in PBS with 15mg/mL BSA and 0.1% sodium azide.|
|Flow Cytometry, Immunocytochemistry|
|Alexa Fluor 647|
|CTNNB1; CTNNB; PRO2286|
|Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag.|
|Protein G Purified|
|Stable for one year at 2°C to 8°C from date of receipt.|
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