AKT1, Mouse, Clone: 18F3.H11, Abnova™

Catalog No.	Quantity
89-108-461	100 μg

Catalog No. 89-108-461 Supplier Abnova Corporation Supplier No. MAB10260

Description

Mouse monoclonal antibody raised against synthetic phosphopeptide of AKT1.

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq

Specifications

• Antigen: AKT1
• Applications: ELISA, Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 18F3.H11
• Conjugate: Unconjugated
• Description: Mouse monoclonal antibody raised against synthetic phosphopeptide of AKT1.
• Dilution: ELISA (1:20000) Western Blot (1:500-1:3000) Immunohistochemistry (20 ug/mL) The optimal working dilution should be determined by the end user.
• Formulation: In 20mM KH₂PO₄, 150mM NaCl, pH 7.2 (0.01% sodium azide)
• Gene: AKT1
• Gene Alias: AKT/MGC99656/PKB/PKB-ALPHA/PRKBA/RAC/RAC-ALPHA
• Gene Symbols: AKT1
• Host Species: Mouse
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding T308 of human AKT1.
• Purification Method: Protein A purification
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 207
• Test Specificity: This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur.
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid