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Mouse anti-Biotin (Vitamin B7 or Vitamin H), Clone: Hyb-8, Azide Free, Novus Biologicals™
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Catalog No. p-5321398 Shop All R&D Systems Products
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0.2 mg
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Catalog No. Quantity
NBP23464602 0.2 mg
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Catalog No. NBP23464602 Supplier Novus Biologicals Supplier No. NBP2346460.2 MG

Description

Mouse Monoclonal Antibody

Biotin (Vitamin B7 or Vitamin H) Monoclonal antibody specifically detects Biotin (Vitamin B7 or Vitamin H) in All Species samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

Antigen Biotin (Vitamin B7 or Vitamin H)
Applications Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone Hyb-8
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
Formulation 10mM PBS - BSA free with No Preservative
Host Species Mouse
Immunogen Biotinylated sheep immunoglobulin
Molecular Weight of Antigen 244 kDa
Purification Method Protein G purified
Quantity 0.2 mg
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Reconstitution Protein A purified
Target Species All species
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1
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