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MilliporeSigma™ Mouse anti-Calpain I/II, large subunit, Clone: 1D10A7,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1215

Catalog No. MABS1215MI


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Description

Description

Specifically detects Calpain I/II, large subunit clone: 1D10A7 in Chicken, Human, Monkey, Rabbit, Rat samples, and it is validated for Immunocytochemistry, Western Blotting

Calpains I & II are intracellular cysteine proteinases distributed in various tissues and cells. Calpain I & II are also known as mu- and m-calpains, respectively, based on their calcium requirement, with calpain I being active at micromolar Ca2+ concentrations and calpain II at millimolar Ca2+ concentrations. Both calpains are composed of a large catalytic (80kDa) and a small regulatory (30kDa) subunit, and each subunit contains a calmodulinlike domain at the C-terminus. Upon Ca2+ binding to the calmodulin-like domains, calpains become activated for catalyzing both substrate proteolytic degradation as well as autolysis. Fourteen calpain large subunits have been identified in human (calpain 1-3 & 5-15 encoded by CAPN1-3 & 5-15 gene) and two small subunits encoded by the CAPNS1 & CAPSN2 genes. The calpain small subunit 1 encoded by CAPNS1 is also known as calpain 4. To date, calpain I & II remain the best characterized members of the calpain family. Calpain activity was initially characterized as 'calcium-activated neutral protease' (CANP) detected in brain, lens of the eye, and other tissues. The name 'calpain' was later adopted as a hybrid name from two well-known proteins at the time, calmodulin and papain.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Calpain I/II, large subunit
Monoclonal
Mouse monoclonal IgG2aκ ascites with 0.05% sodium azide.
CAPN1; CANPL1; PIG30
Purified m-calpain from rabbit skeletal muscle.
50 μL
Signaling
NP_001185797
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
IgG2a κ
Immunocytochemistry, Western Blot
1D10A7
P07384
Mouse
Unpurified
RUO
Primary
Chicken, Human, Monkey, Rabbit, Rat
Ascites
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