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MilliporeSigma™ Mouse anti-Calpain I/II, large subunit, Clone: 1D10A7,
Mouse Monoclonal Antibody
Supplier: MilliporeSigma™ MABS1215
Description
Specifically detects Calpain I/II, large subunit clone: 1D10A7 in Chicken, Human, Monkey, Rabbit, Rat samples, and it is validated for Immunocytochemistry, Western Blotting
Calpains I & II are intracellular cysteine proteinases distributed in various tissues and cells. Calpain I & II are also known as mu- and m-calpains, respectively, based on their calcium requirement, with calpain I being active at micromolar Ca2+ concentrations and calpain II at millimolar Ca2+ concentrations. Both calpains are composed of a large catalytic (80kDa) and a small regulatory (30kDa) subunit, and each subunit contains a calmodulinlike domain at the C-terminus. Upon Ca2+ binding to the calmodulin-like domains, calpains become activated for catalyzing both substrate proteolytic degradation as well as autolysis. Fourteen calpain large subunits have been identified in human (calpain 1-3 & 5-15 encoded by CAPN1-3 & 5-15 gene) and two small subunits encoded by the CAPNS1 & CAPSN2 genes. The calpain small subunit 1 encoded by CAPNS1 is also known as calpain 4. To date, calpain I & II remain the best characterized members of the calpain family. Calpain activity was initially characterized as 'calcium-activated neutral protease' (CANP) detected in brain, lens of the eye, and other tissues. The name 'calpain' was later adopted as a hybrid name from two well-known proteins at the time, calmodulin and papain.
Specifications
Calpain I/II, large subunit | |
Monoclonal | |
Mouse monoclonal IgG2aκ ascites with 0.05% sodium azide. | |
CAPN1; CANPL1; PIG30 | |
Purified m-calpain from rabbit skeletal muscle. | |
50 μL | |
Signaling | |
NP_001185797 | |
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance. | |
IgG2a κ |
Immunocytochemistry, Western Blot | |
1D10A7 | |
P07384 | |
Mouse | |
Unpurified | |
RUO | |
Primary | |
Chicken, Human, Monkey, Rabbit, Rat | |
Ascites |
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