CD117 Mouse anti-Human, APC-R700, Clone: YB5.B8, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 565195
The YB5.B8 monoclonal antibody specifically binds to CD117, which is also known as c-Kit, or Stem cell factor Receptor (SCFR). CD117 is a ~145 kDa type I transmembrane glycoprotein with tyrosine kinase activity. CD117 is present on hematopoietic progenitor cell subsets, thymocytes, mast cells, hepatocytes and histiocytes. CD117 serves as a cytokine receptor for steel factor (SLF), also known as stem cell factor (SCF), or mast cell growth factor (MGF). The interaction of CD117 and SLF is crucial to hematopoiesis, mast cell differentiation, melanogenesis, and germ cell development. The ability of the YB5.B8 antibody to block the binding of c-Kit ligand is still controversial.
This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor (eg, 730/45-nm filter).
|KIT; c-Kit; SCFR; PBT; Mast/stem cell growth factor receptor|
|Acute myelocytic leukemia blasts|
|Aqueous buffered solution containing BSA, protein stabilizer, glycerol, and ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|